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Rabbit anti-AF6 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
AF6
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 1774 and 1824
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Rat, Mouse
Antigen Species
Human
Stock Concentration
400 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to AF6 immobilized on solid support.

The epitope recognized by A302-200A-T maps to a region between residue 1774 and 1824 of human AF6 (myeloid/lymphoid or mixed-lineage leukemia translocated to, 4 ) using the numbering given in entry BAA32484.1 (GeneID 4301).

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Gene & Protein Information

AF6 was identified as part of a fusion with MLL (mixed lineage leukemia) in a t(6;11) genetic translocation. AF6 is also known as afadin (a splicing variant of AF6 found at adherins junction). AF6/afadin is an actin filament binding protein that binds to nectin at cadherin-based cell-cell adherens junctions and regulates cell-cell adhesion by mediating Ras signaling.

4301

AFDN

afadin, adherens junction formation factor

P55196

Afadin

Alternate Names

adherens junction formation factor, AF6, afadin, ALL1-fused gene from chromosome 6 protein, l-afadin, MLL-AF6, MLLT4, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4, protein AF-6

Applications

Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.