Bethyl Laboratories
Rabbit anti-AF6 Antibody Affinity Purified
Primary
Catalog #A302-200A
TargetAF6
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Image 1 of 1
local_shipping
Order within 5 hours and 18 minutes for shipment by Tuesday, November 17
Product Description
AF6 was identified as part of a fusion with MLL (mixed lineage leukemia) in a t(6;11) genetic translocation. AF6 is also known as afadin (a splicing variant of AF6 found at adherins junction). AF6/afadin is an actin filament binding protein that binds to nectin at cadherin-based cell-cell adherens junctions and regulates cell-cell adhesion by mediating Ras signaling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1774 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAF6
Antigen Species
Human
Gene ID4301
Gene Symbol
AFDN
Gene Name
afadin, adherens junction formation factor
Uniprot IDP55196
Protein Name
Afadin
Alternate Names
adherens junction formation factor • AF6 • afadin • ALL1-fused gene from chromosome 6 protein • l-afadin • MLL-AF6 • MLLT4 • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) • translocated to, 4 • myeloid/lymphoid or mixed-lineage leukemia • translocated to, 4 • protein AF-6
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AF6 immobilized on solid support.
The epitope recognized by A302-200A maps to a region between residue 1774 and 1824 of human AF6 (myeloid/lymphoid or mixed-lineage leukemia translocated to, 4 ) using the numbering given in entry BAA32484.1 (GeneID 4301).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A302-200A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-200A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-200AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1