Bethyl Laboratories
Rabbit anti-AF9 Antibody, Affinity Purified
Primary
Catalog #A300-595A
TargetAF9
HostRabbit
ClonalityPolyclonal
ApplicationIP, WB
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Product Description
The human AF9 gene is one of the most common fusion partner genes with the MLL (mixed lineage leukemia) gene resulting in AF9/MLL translocations and leukemogenesis. AF9 is highly homologous to the gene product of ENL, another common MLL fusion partner gene. The function of AF9 is not known but through studies of ENL it is proposed to be a nuclear transactivating factor.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAF9
Antigen Species
Human
Gene ID4300
Gene Symbol
MLLT3
Gene Name
MLLT3, super elongation complex subunit
Uniprot IDP42568
Protein Name
Protein AF-9
Alternate Names
AF9 • ALL1-fused gene from chromosome 9 protein • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog) • translocated to, 3 • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) • translocated to, 3 • myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein • myeloid/lymphoid or mixed-lineage leukemia • translocated to 3 • protein AF-9 • YEATS domain-containing protein 3 • YEATS3
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AF9 immobilized on solid support.
The epitope recognized by A300-595A maps to a region between residues 200 and 250 of human ALL1 Fused Gene from Chromosome 9 Protein (MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocate 3) using the numbering given in entry NP_004520.1 (GeneID 4300).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A300-595A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-595A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-595AOpen
Citations / Publications
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