Bethyl Laboratories
Rabbit anti-AIF Antibody Affinity Purified
Primary
Catalog #A302-783A
TargetAIF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Apoptosis-inducing factor (AIF) is a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, AIF induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 563 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAIF
Antigen Species
Human
Gene ID9131
Gene Symbol
AIFM1
Gene Name
apoptosis inducing factor, mitochondria associated 1
Uniprot IDO95831
Protein Name
Apoptosis-inducing factor 1, mitochondrial
Alternate Names
AIF • apoptosis-inducing factor 1, mitochondrial • apoptosis-inducing factor, mitochondrion-associated, 1 • CMT2D • CMTX4 • COWCK • COXPD6 • DFNX5 • NADMR • NAMSD • PDCD8 • programmed cell death 8 (apoptosis-inducing factor) • Programmed cell death protein 8 • striatal apoptosis-inducing factor • testicular secretory protein Li 4
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AIF immobilized on solid support.
The epitope recognized by A302-783A maps to a region between residue 563 and 613 of human Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 using the numbering given in entry NP_004199.1 (GeneID 9131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-783A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-783A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-783AOpen
Citations / Publications
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