Bethyl Laboratories
Rabbit anti-AKAP13/AKAP-Lbc Antibody Affinity Purified
Primary
Catalog #A301-403A
TargetAKAP13/AKAP-Lbc
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
AKAP13/AKAP-Lbc was originally identified as an oncogenic protein that associates with the GTP-binding protein Rho. AKAP13-Lbc is also a member of the A-kinase anchoring protein family that functions as a molecular scaffold for the subcellular compartmentalization of A- kinase signaling molecules. AKAP13-Lbc has been found to possess Rho-specific guanine nucleotide exchange factor (GEF) activity and is critical to mediating RhoA activation and signal transduction.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1850 and 1900
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAKAP13/AKAP-Lbc
Antigen Species
Human
Gene ID11214
Gene Symbol
AKAP13
Gene Name
A-kinase anchoring protein 13
Uniprot IDQ12802
Protein Name
A-kinase anchor protein 13
Alternate Names
A kinase (PRKA) anchor protein 13 • AKAP-13 • AKAP-Lbc • A-kinase anchor protein 13 • ARHGEF13 • breast cancer nuclear receptor-binding auxiliary protein • BRX • c-lbc • guanine nucleotide exchange factor Lbc • HA-3 • Ht31 • human thyroid-anchoring protein 31 • LBC • LBC oncogene • lymphoid blast crisis oncogene • non-oncogenic Rho GTPase-specific GTP exchange factor • p47 • PRKA13 • protein kinase A-anchoring protein 13 • PROTO-LB • PROTO-LBC
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKAP13/AKAP-Lbc immobilized on solid support.
The epitope recognized by A301-403A maps to a region between residue 1850 and 1900 of human A kinase (PRKA) anchor protein 13 using the numbering given in entry NP_009131.2 (GeneID 11214).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-403A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-403A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-403AOpen
Citations / Publications
Services
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