Bethyl Laboratories
Rabbit anti-AKAP8/AKAP95 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00217
TargetAKAP8/AKAP95
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
AKAP8/AKAP95 has been characterized as a 95 kDa protein kinase A (PKA)-anchoring protein and regulatory subunit of PKA that functions to recruit the condensin complex for chromosome condensation during mitosis. A role in DNA replication has also been demonstrated through its interaction with the pre-replication complex component, MCM2 (mini-chromosome maintenance 2). Inhibition of AKAP8/AKAP95 association with MCM2 inhibits replication initiation and elongation in vitro. AKAP8/AKAP95 has also been shown to be involved in apoptosis. KAP8/AKAP95 associates with caspase 3 and serves as a carrier to transport caspase 3 from the cytoplasm to the nucleus to induce morphological changes in the nucleus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 642 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetAKAP8/AKAP95
Antigen Species
Human
Gene ID10270
Gene Symbol
AKAP8
Gene Name
A-kinase anchoring protein 8
Uniprot IDO43823
Protein Name
A-kinase anchor protein 8
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKAP8/AKAP95 immobilized on solid support.
The epitope recognized by IHC-00217 maps to a region between residue 642 and the C-terminus (residue 692) of human A-kinase anchor protein 8 using the numbering given in entry NP_005849.1 (GeneID 10270).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00217Open
Product DatasheetPDF · IHC-00217 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00217 · Lot 1 (current lot)Open
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