/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-AKAP8L/HA95 Antibody
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Primary Antibodies  

Rabbit anti-AKAP8L/HA95 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

AKAP8L/HA95

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 525 and 575

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to AKAP8L/HA95 immobilized on solid support.

The epitope recognized by A301-365A maps to a region between residue 525 and 575 of human A kinase (PRKA) anchor protein 8-like (helicase A-binding protein 95 kDa) using the numbering given in entry NP_055186.2 (GeneID 26993).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to AKAP8L/HA95 immobilized on solid support.

The epitope recognized by A301-365A-T maps to a region between residue 525 and 575 of human A kinase (PRKA) anchor protein 8-like (helicase A-binding protein 95 kDa) using the numbering given in entry NP_055186.2 (GeneID 26993).

Additional Product Information

AKAP8L/HA95 is a PKA anchoring protein that is localized to the nuclear envelope and associates with chromatin upon nuclear envelope breakdown during mitosis. AKAP8L/HA95 has been found to play an important role in the initiation of DNA replication and may function as a scaffold for proteins such as MCM2 and LAP2beta. Additionally, AKAP8L/HA95 has been shown to co-locate with the PKA C subunit in splicing factor compartments and is involved in the regulation of pre-mRNA splicing. Further support for a scaffolding and splicing role in the nucleus comes from the observation that AKAP8L/HA95 colocalizes with HypA, a splicing-like factor, and through this association may function as a docking site for the nuclear accumulation of Huntington protein as seen in Huntington’s disease.

26993

AKAP8L

A-kinase anchoring protein 8 like

Q9ULX6

A-kinase anchor protein 8-like

Alternate Names

A kinase (PRKA) anchor protein 8-like; AKAP8-like protein; A-kinase anchor protein 8-like; HA95; HAP95; Helicase A-binding protein 95; helicase A-binding protein 95 kDa; homologous to AKAP95 protein; NAKAP; NAKAP95; neighbor of AKAP95; neighbor of A-kinase anchoring protein 95; Neighbor of A-kinase-anchoring protein 95; testis tissue sperm-binding protein Li 90mP

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.