Bethyl Laboratories
Rabbit anti-AKAP8L/HA95 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00277
TargetAKAP8L/HA95
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
AKAP8L/HA95 is a PKA anchoring protein that is localized to the nuclear envelope and associates with chromatin upon nuclear envelope breakdown during mitosis. AKAP8L/HA95 has been found to play an important role in the initiation of DNA replication and may function as a scaffold for proteins such as MCM2 and LAP2beta. Additionally, AKAP8L/HA95 has been shown to co-locate with the PKA C subunit in splicing factor compartments and is involved in the regulation of pre-mRNA splicing. Further support for a scaffolding and splicing role in the nucleus comes from the observation that AKAP8L/HA95 colocalizes with HypA, a splicing-like factor, and through this association may function as a docking site for the nuclear accumulation of Huntington protein as seen in Huntington’s disease.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 596 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAKAP8L/HA95
Antigen Species
Human
Gene ID26993
Gene Symbol
AKAP8L
Gene Name
A-kinase anchoring protein 8 like
Uniprot IDQ9ULX6
Protein Name
A-kinase anchor protein 8-like
Alternate Names
A kinase (PRKA) anchor protein 8-like • AKAP8-like protein • A-kinase anchor protein 8-like • HA95 • HAP95 • Helicase A-binding protein 95 • helicase A-binding protein 95 kDa • homologous to AKAP95 protein • NAKAP • NAKAP95 • neighbor of AKAP95 • neighbor of A-kinase anchoring protein 95 • Neighbor of A-kinase-anchoring protein 95 • testis tissue sperm-binding protein Li 90mP
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKAP8L/HA95 immobilized on solid support.
The epitope recognized by IHC-00277 maps to a region between residue 596 and 646 of human A kinase (PRKA) anchor protein 8-like (helicase A-binding protein 95 kDa) using the numbering given in entry NP_055186.2 (GeneID 26993).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00277Open
Product DatasheetPDF · IHC-00277 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00277 · Lot 1 (current lot)Open
Services
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