Bethyl Laboratories
Rabbit anti-AktS1/PRAS40 Antibody Affinity Purified
Primary
Catalog #A301-201A
TargetAktS1/PRAS40
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
AktS1/PRAS40 interacts with raptor as a component of the mammalian target of rapamycin complex 1 (mTORC1). One study suggests that AktS1/PRAS40 may function to regulate mTORC1 kinase activity by inhibiting substrate binding. Another study suggests that AktS1/PRAS40 functions downstream of mTORC1 and upstream from its effectors S6K1, and 4E-BP1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 206 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAktS1/PRAS40
Antigen Species
Human
Gene ID84335
Gene Symbol
AKT1S1
Gene Name
AKT1 substrate 1
Uniprot IDQ96B36
Protein Name
Proline-rich AKT1 substrate 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AktS1/PRAS40 immobilized on solid support.
The epitope recognized by A301-201A maps to a region between residue 195 and 245 of human AKT1 substrate 1 (proline-rich Akt substrate, 40 kDa) using the numbering given in entry NP_115751.1 (GeneID 84335).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-201A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-201A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-201AOpen
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