Bethyl Laboratories
Rabbit anti-ALIX Antibody Affinity Purified
Primary
Catalog #A302-938A
TargetALIX
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
ALIX (ALG-2 interacting protein X) is thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of ALIX can block apoptosis. ALIX has been shown to bind to the PDCD6 (programmed cell death 6 interacting protein), a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis [taken from NCBI Entrez Gene (Gene ID: 10015].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 818 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetALIX
Antigen Species
Human
Gene ID10015
Gene Symbol
PDCD6IP
Gene Name
programmed cell death 6 interacting protein
Uniprot IDQ8WUM4
Protein Name
Programmed cell death 6-interacting protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ALIX immobilized on solid support.
The epitope recognized by A302-938A maps to a region between residue 818 and 868 of human Apoptosis-Linked Gene 2-Interacting Protein X using the numbering given in entry NP_037506.2 (GeneID 10015).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-938A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-938A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-938AOpen
Citations / Publications
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