Bethyl Laboratories
Rabbit anti-Alpha-4 Antibody Affinity Purified
Primary
Catalog #A300-470A
TargetAlpha-4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Alpha-4 is involved in the rapamycin sensitive mTOR pathway through its association with the PP2Ac phosphatase. Alpha-4 may regulate PP2Ac activity for the cell cycle progression of lymphocytes. Alpha-4 is also associated to surface IgM receptors and is involved in B-cell receptor mediated signal transduction.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAlpha-4
Antigen Species
Human
Gene ID3476
Gene Symbol
IGBP1
Gene Name
immunoglobulin (CD79A) binding protein 1
Uniprot IDP78318
Protein Name
Immunoglobulin-binding protein 1
Alternate Names
ALPHA-4 • B cell signal transduction molecule alpha 4 • bA351K23.1 (immunoglobulin binding protein 1 (CD79A) ) • B-cell signal transduction molecule alpha 4 • CD79a-binding protein 1 • IBP1 • immunoglobulin (CD79A) binding protein 1 • immunoglobulin-binding protein 1 • protein alpha-4 • protein phosphatase 2/4/6 regulatory subunit • protein phosphatase 2A, regulatory subunit alpha-4 • renal carcinoma antigen NY-REN-16
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Alpha-4 (IGBP1) immobilized on solid support.
The epitope recognized by A300-470A maps to a region between residues 200 and 250 of human Protein Phosphatase 2A, regulatory subunit alpha-4 (Immunoglobulin binding protein 1) using the numbering given in SwissProt entry P78318 (GeneID 3476).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-470A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-470A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-470AOpen
Services
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