Bethyl Laboratories

Rabbit anti-ANKS3 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00285
TargetANKS3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
Detection of human ANKS3 by immunohistochemistry.
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Product Description

ANKS3 (ankyrin repeat and SAM domain-containing protein 3) contains 6 ANK (ankyrin) repeats and a SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. The function of ANKS3 has not been characterized.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 606 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetANKS3
Antigen Species
Human
Gene ID124401
Gene Symbol
ANKS3
Gene Name
ankyrin repeat and sterile alpha motif domain containing 3
Uniprot IDQ6ZW76
Protein Name
Ankyrin repeat and SAM domain-containing protein 3
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to ANKS3 immobilized on solid support.

The epitope recognized by IHC-00285 maps to a region between residue 606 and 656 of human ankyrin repeat and sterile alpha motif domain containing 3 using the numbering given in entry NP_597707.1 (GeneID 124401).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00285Open
Product DatasheetPDF · IHC-00285 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00285 · Lot 1 (current lot)Open
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