Bethyl Laboratories
Rabbit anti-Ankyrin 1 Antibody Affinity Purified
Primary
Catalog #A303-048A
TargetAnkyrin 1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Ankyrin 1 is a member of the ankyrin family of proteins that function to link integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Ankyrin 1 was first discovered in erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis [taken from NCBI Entrez Gene (Gene ID: 286].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1831 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAnkyrin 1
Antigen Species
Human
Gene ID286
Gene Symbol
ANK1
Gene Name
ankyrin 1
Uniprot IDP16157
Protein Name
Ankyrin-1
Alternate Names
ANK • ANK-1 • ankyrin 1, erythrocytic • ankyrin-1 • ankyrin-R • erythrocyte ankyrin • SPH1 • SPH2
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Ankyrin 1 immobilized on solid support.
The epitope recognized by A303-048A maps to a region between residue 1831 and 1881 of human Ankyrin 1 using the numbering given in entry NP_065209.2 (GeneID 286).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A303-048A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-048A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-048AOpen
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