Bethyl Laboratories
Rabbit anti-APC1 Antibody Affinity Purified
Primary
Catalog #A301-653A
TargetAPC1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Anaphase-promoting complex subunit 1(APC1) is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase and is responsible for the ubiquitination of securin and cyclin B and prompts the onset of anaphase and the exit from mitosis. The APC complex is composed of at least 11 APC components. APC1 is the largest of these subunits.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAPC1
Antigen Species
Human
Gene ID64682
Gene Symbol
ANAPC1
Gene Name
anaphase promoting complex subunit 1
Uniprot IDQ9H1A4
Protein Name
Anaphase-promoting complex subunit 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to APC1 immobilized on solid support.
The epitope recognized by A301-653A maps to a region between residue 500 and 550 of human anaphase promoting complex subunit 1 using the numbering given in entry NP_073153.1 (GeneID 64682).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-653A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-653A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-653AOpen
Citations / Publications
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