Bethyl Laboratories
Rabbit anti-APC7 Antibody Affinity Purified
Primary
Catalog #A302-550A
TargetAPC7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
Anaphase promoting complex subunit 7 (APC7) is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B which prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAPC7
Antigen Species
Human
Gene ID51434
Gene Symbol
ANAPC7
Gene Name
anaphase promoting complex subunit 7
Uniprot IDQ9UJX3
Protein Name
Anaphase-promoting complex subunit 7
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to APC7 immobilized on solid support.
The epitope recognized by A302-550A maps to a region between residue 75 and 125 of human anaphase promoting complex subunit 7 using the numbering given in entry AAI41849.1 (GeneID 51434).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-550A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-550A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-550AOpen
Citations / Publications
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