Rabbit anti-ArfGAP3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-032A-T maps to a region between residue 466 and 516 of human ADP-ribosylation factor GTPase activating protein 3 using the numbering given in entry NP_055385.2 (GeneID 26286). Antibody was affinity purified using an epitope specific to ArfGAP3 immobilized on solid support.
The epitope recognized by A302-032A maps to a region between residue 466 and 516 of human ADP-ribosylation factor GTPase activating protein 3 using the numbering given in entry NP_055385.2 (GeneID 26286).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ADP-ribosylation factor GTPase activating protein 3 (ArfGAP3) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. Although they are differentially regulated, ArfGAP3 along with ArfGAP1 and ArfGAP2 has been shown to play an overlapping role in COPI-mediated transport and in the maintenance of Golgi organization.
Alternate Names
ADP-ribosylation factor GTPase activating protein 1; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFGAP1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.