Bethyl Laboratories
Rabbit anti-ARHGEF16 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00582
TargetARHGEF16
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
The gene for ARHGEF16 [Rho guanine exchange factor (GEF)16] was isolated as a candidate gene for human neuroblastoma. It is a member of the Rho/Rac GEF family of proteins which catalyze the exchange of GDP for GTP on the GTPases, Rho and Rac, to activate downstream signaling pathways.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetARHGEF16
Antigen Species
Human
Gene ID27237
Gene Symbol
ARHGEF16
Gene Name
Rho guanine nucleotide exchange factor 16
Uniprot IDQ5VV41
Protein Name
Rho guanine nucleotide exchange factor 16
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ARHGEF16 immobilized on solid support.
The epitope recognized by IHC-00582 maps to a region between residue 175 and 225 of human Rho guanine exchange factor (GEF) 16 using the numbering given in entry NP_055263.2 (GeneID 27237).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00582Open
Product DatasheetPDF · IHC-00582 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00582 · Lot 1 (current lot)Open
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