/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ARID1B Antibody
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Primary Antibodies  

Rabbit anti-ARID1B Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

ARID1B

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1400 and 1450

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ARID1B immobilized on solid support.

The epitope recognized by A301-046A maps to a region between residue 1300 and 1350 of human AT rich interactive domain 1B using the numbering given in entry NP_059989.2 (GeneID 57492).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ARID1B immobilized on solid support.

The epitope recognized by A301-046A-T maps to a region between residue 1300 and 1350 of human AT rich interactive domain 1B using the numbering given in entry NP_059989.2 (GeneID 57492).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1B is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1B is encoded by a gene independent from ARID1A and exhibits functionally distinct characteristics.

57492

ARID1B

AT-rich interaction domain 1B

Q8NFD5

AT-rich interactive domain-containing protein 1B

Alternate Names

6A3-5;ARID domain-containing protein 1B;AT rich interactive domain 1B (SWI1-like);AT-rich interactive domain-containing protein 1B;BAF250B;BRG1-associated factor 250b;BRG1-binding protein ELD/OSA1;BRG1-binding protein hELD/OSA1;BRIGHT;CSS1;DAN15;ELD (eyelid)/OSA protein;ELD/OSA1;hOsa2;MRD12;Osa homolog 2;OSA2;P250R

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.