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Rabbit anti-ARID1B Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
ARID1B
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 1300 and 1350
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
100 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to ARID1B immobilized on solid support.

The epitope recognized by A301-046A-T maps to a region between residue 1300 and 1350 of human AT rich interactive domain 1B using the numbering given in entry NP_059989.2 (GeneID 57492).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1B is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1B is encoded by a gene independent from ARID1A and exhibits functionally distinct characteristics.

57492

ARID1B

AT-rich interaction domain 1B

Q8NFD5

AT-rich interactive domain-containing protein 1B

Alternate Names

6A3-5, ARID domain-containing protein 1B, AT rich interactive domain 1B (SWI1-like), AT-rich interactive domain-containing protein 1B, BAF250B, BRG1-associated factor 250b, BRG1-binding protein ELD/OSA1, BRG1-binding protein hELD/OSA1, BRIGHT, CSS1, DAN15, ELD (eyelid)/OSA protein, ELD/OSA1, hOsa2, MRD12, Osa homolog 2, OSA2, P250R

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Citations (2)