Bethyl Laboratories
Rabbit anti-ARID4A Antibody Affinity Purified
Primary
Catalog #A302-231A
TargetARID4A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
ARID4A (AT-rich interactive domain-containing protein 4A) is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4A also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. ARID4A is implicated to be involved in chromatin remodeling and epigenetic modifications and to play a role in the pathogenesis of cancer.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 925 and 975
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetARID4A
Antigen Species
Human
Gene ID5926
Gene Symbol
ARID4A
Gene Name
AT-rich interaction domain 4A
Uniprot IDP29374
Protein Name
AT-rich interactive domain-containing protein 4A
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ARID4A immobilized on solid support.
The epitope recognized by A302-231A maps to a region between residue 900 and 950 of human AT-rich interactive domain-containing protein 4A using the numbering given in entry NP_002883.3 (GeneID 5926).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-231A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-231A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-231AOpen
Citations / Publications
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