Bethyl Laboratories
Rabbit anti-ARPC4 Antibody Affinity Purified
Primary
Catalog #A305-385A
TargetARPC4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Actin-related protein 2/3 complex subunit 4 (ARPC4) functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC4 seems to contact the mother actin filament [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P59998].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 118 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetARPC4
Antigen Species
Human
Gene ID10093
Gene Symbol
ARPC4
Gene Name
actin related protein 2/3 complex subunit 4
Uniprot IDP59998
Protein Name
Actin-related protein 2/3 complex subunit 4
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ARPC4 immobilized on solid support.
The epitope recognized by A305-385A maps to a region between residue 118 to 168 of human using the numbering given in entry P59998.3 (GeneID 10093).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-385A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-385A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-385AOpen
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Resources
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