Bethyl Laboratories
Rabbit anti-Artemis IHC Antibody Affinity Purified
Primary
Catalog #IHC-00408
TargetArtemis
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Artemis is also known as DNA cross-link repair 1C protein (DCLRE1C) and functions as both an endo- and exo-nuclease involved in V(D)J recombination and double-strand break (DSB) repair. In addition to repair of double stranded breaks during V(D)J recombination, Artemis may also be required for repair of ionizing radiation induced DSBs. Mutations in Artemis have been found to be a cause of severe combined immunodeficiency (SCID).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 642 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetArtemis
Antigen Species
Human
Gene ID64421
Gene Symbol
DCLRE1C
Gene Name
DNA cross-link repair 1C
Uniprot IDQ96SD1
Protein Name
Protein artemis
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Artemis immobilized on solid support.
The epitope recognized by IHC-00408 maps to a region between residues 650 and the C-terminus (residue 692) of human DNA cross-link repair 1C (aka Artemis) using the numbering given in TrEMBL entry Q96SD1 (GeneID 64421).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00408Open
Product DatasheetPDF · IHC-00408 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00408 · Lot 1 (current lot)Open
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