/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ASH2 Antibody
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Primary Antibodies  

Rabbit anti-ASH2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (43)
Target:

ASH2

Reactivity:

Human

Applications:

ChIP

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 578 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ASH2 immobilized on solid support.

The epitope recognized by A300-489A maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using the numbering given in Swiss-Prot entry Q9UBL3 (GeneID 9070).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

ASH2 (absent, small, or homeotic discs 2) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. ASH2 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX.

9070

ASH2L

ASH2 like, histone lysine methyltransferase complex subunit

Q9UBL3

Set1/Ash2 histone methyltransferase complex subunit ASH2

Alternate Names

ASH2; ash2 (absent, small, or homeotic)-like; ASH2L1; ASH2L2; ASH2-like protein; Bre2; set1/Ash2 histone methyltransferase complex subunit ASH2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.