Bethyl Laboratories
Rabbit anti-ASH2 Antibody Affinity Purified
Primary
Catalog #A300-489A
TargetASH2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationChIP, IHC, IP, WB
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Product Description
ASH2 (absent, small, or homeotic discs 2) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. ASH2 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 578 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetASH2
Antigen Species
Human
Gene ID9070
Gene Symbol
ASH2L
Gene Name
ASH2 like histone lysine methyltransferase complex subunit
Uniprot IDQ9UBL3
Protein Name
Set1/Ash2 histone methyltransferase complex subunit ASH2
Alternate Names
Product Information
Applications
ChIP, IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ASH2 immobilized on solid support.
The epitope recognized by A300-489A maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using the numbering given in Swiss-Prot entry Q9UBL3 (GeneID 9070).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Chromatin IP (ChIP)1 - 5 µg. Previous lots of this antibody have performed in this application.
ChIP-chip10 µg. Previous lots of this antibody have performed in this application.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:20,000
Documents
Product DatasheetPDF · A300-489A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-489A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-489AOpen
Citations / Publications
Services
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