Bethyl Laboratories
Rabbit anti-ASPH Antibody Affinity Purified
Primary
Catalog #A305-343A
TargetASPH
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Aspartyl/asparaginyl beta-hydroxyl (ASPH) Isoform 1 specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12797].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 708 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetASPH
Antigen Species
Human
Gene ID444
Gene Symbol
ASPH
Gene Name
aspartate beta-hydroxylase
Uniprot IDQ12797
Protein Name
Aspartyl/asparaginyl beta-hydroxylase
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ASPH immobilized on solid support.
The epitope recognized by A305-343A maps to a region between residue 708 to 758 of human Aspartyl/asparaginyl beta-hydroxylase using the numbering given in entry Q12797.3 (GeneID 444).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-343A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-343A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-343AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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