Bethyl Laboratories
Rabbit anti-ASPP1 Antibody Affinity Purified
Primary
Catalog #A300-918A
TargetASPP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The Apoptosis-Stimulating Protein of P53 (ASPP) family of proteins is comprised of ASPP1, ASPP2 and iASPP. 53BP2 is now recognized to be the C-terminal 528 amino acids of ASPP2. ASPP1 and ASPP2 function to direct the cell away from cell cycle arrest and towards death by activating p53 family members, while iASPP functions to inhibit p53 mediated apoptosis. ASPP proteins have been shown to interact with p53, Bcl-2, and ReLA/p65.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetASPP1
Antigen Species
Human
Gene ID23368
Gene Symbol
PPP1R13B
Gene Name
protein phosphatase 1 regulatory subunit 13B
Uniprot IDQ96KQ4
Protein Name
Apoptosis-stimulating of p53 protein 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ASPP1 immobilized on solid support.
The epitope recognized by A300-918A maps to a region between residue 475 and 525 of human Apoptosis-Stimulated Protein of p53, 1 using the numbering given in entry NP_056131.1 (GeneID 23368).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A300-918A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-918A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-918AOpen
Citations / Publications
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