Bethyl Laboratories
Rabbit anti-Astrin/MAP126 Antibody Affinity Purified
Primary
Catalog #A301-511A
TargetAstrin/MAP126
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 23 hours and 17 minutes for shipment by today
Product Description
Astrin/MAP126 has been identified as a mitotic spindle-associated protein essential for centrosome integrity and the progression through mitosis. Astrin/MAP126 interacts with Ninein and Aurora-A at the centrosome. Ninein, but not Aurora-A, appears to be required for proper localization of Astrin/MAP126 to the centrosome during S and G2 phase. A pool of Astrin/MAP126 also localizes to the outer kinetochore where it plays an essential role in chromatid adhesion and chromosome congression and segregation. The localization of Astrin/MAP126 to the spindle pole and kinetochore has been shown to be dependent on its phosphorylation by glycogen synthase kinase 3beta (GSK3beta).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAstrin/MAP126
Antigen Species
Human
Gene ID10615
Gene Symbol
SPAG5
Gene Name
sperm associated antigen 5
Uniprot IDQ96R06
Protein Name
Sperm-associated antigen 5
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Astrin/MAP126 immobilized on solid support.
The epitope recognized by A301-511A maps to a region between residue 1 and 50 of human astrin (mitotic spindle associated protein p126) using the numbering given in entry NP_006452.3 (GeneID 10615).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-511A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-511A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-511AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1