Bethyl Laboratories
Rabbit anti-ATF1 Antibody Affinity Purified
Primary
Catalog #A303-035A
TargetATF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
ATF1 (activating transcription factor 1) is a transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetATF1
Antigen Species
Human
Gene ID466
Gene Symbol
ATF1
Gene Name
activating transcription factor 1
Uniprot IDP18846
Protein Name
Cyclic AMP-dependent transcription factor ATF-1
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ATF1 immobilized on solid support.
The epitope recognized by A303-035A maps to a region between residue 171 and 221 of human Activating Transcription Factor 1 using the numbering given in entry NP_005162.1 (GeneID 466).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-035A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-035A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-035AOpen
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