Bethyl Laboratories
Rabbit anti-Atg4B Antibody Affinity Purified
Primary
Catalog #A302-898A
TargetAtg4B
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Atg4B (ATG4 autophagy related 4 homolog B) is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9Y4P1].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 343 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAtg4B
Antigen Species
Human
Gene ID23192
Gene Symbol
ATG4B
Gene Name
autophagy related 4B cysteine peptidase
Uniprot IDQ9Y4P1
Protein Name
Cysteine protease ATG4B
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Atg4B immobilized on solid support.
The epitope recognized by A302-898A maps to a region between residue 350 and 400 of human Autophagy-related Protein 4 Homolog B using the numbering given in entry NP_037457.3 (GeneID 23192).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-898A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-898A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-898AOpen
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