Bethyl Laboratories
Rabbit anti-ATP5H Antibody Affinity Purified
Primary
Catalog #A305-491A
TargetATP5H
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H is the d subunit of the Fo complex [taken from NCBI Entrez Gene (Gene ID: 10476)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 111 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetATP5H
Antigen Species
Human
Gene ID10476
Gene Symbol
ATP5PD
Gene Name
ATP synthase, H+ transporting, mitochondrial Fo complex subunit D
Uniprot IDO75947
Protein Name
ATP synthase subunit d, mitochondrial
Alternate Names
APT5H • ATP synthase D chain, mitochondrial • ATP synthase peripheral stalk subunit d • ATP synthase subunit d, mitochondrial • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d • ATP synthase, H+ transporting, mitochondrial F1F0, subunit d • ATP synthase, H+ transporting, mitochondrial Fo complex subunit D • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d • ATP5H • ATPase subunit d • ATPQ • My032 protein
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ATP5H immobilized on solid support.
The epitope recognized by A305-491A maps to a region between residue 100 to 150 of human ATP synthase subunit d, mitochondrial using the numbering given in entry O75947.3 (GeneID 10476).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-491A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-491A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-491AOpen
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