Bethyl Laboratories
Rabbit anti-Aurora B IHC Antibody Affinity Purified
Primary
Catalog #IHC-00003
TargetAurora B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC
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Product Description
Aurora B is the central enzymatic unit of the chromosomal passenger complex (CPC). In the CPC, Aurora B is associated with the passenger proteins inner centromeric protein (INCENP), survivin, and borealin. Aurora B is a serine-threonine protein kinase essential for chromosome segregation and cytokinesis. Specific roles for Aurora B have been demonstrated in cohesin dissociation from centromeres as well as condensin I binding to chromatin.F112. Substrates of Aurora B kinase activity include desmin, septin 1, INCENP, RAC GTPase activating protein 1, and centromeric protein A.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 294 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetAurora B
Antigen Species
Human
Gene ID9212
Gene Symbol
AURKB
Gene Name
aurora kinase B
Uniprot IDQ96GD4
Protein Name
Aurora kinase B
Alternate Names
AIK2 • AIM1 • AIM-1 • ARK2 • ARK-2 • AurB • aurkb-sv1 • aurkb-sv2 • Aurora 1 • aurora- and Ipl1-like midbody-associated protein 1 • aurora kinase B • aurora kinase B-Sv1 • aurora kinase B-Sv2 • aurora/IPL1-related kinase 2 • aurora-1 • aurora-B • aurora-related kinase 2 • IPL1 • PPP1R48 • protein phosphatase 1, regulatory subunit 48 • serine/threonine kinase 12 • serine/threonine-protein kinase 12 • serine/threonine-protein kinase 5 • serine/threonine-protein kinase aurora-B • STK-1 • STK12 • STK5
Product Information
Applications
ICC-IF, IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Aurora B immobilized on solid support.
The epitope recognized by IHC-00003 maps to a region between residue 300 and the C-terminus (residue 344) of human Aurora B using the numbering given in SwissProt entry Q96GD4 (GeneID 9212).
Applications
Formalin-fixed Paraffin-Embedded Tissue 1:100 - 1:500:
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Formalin-fixed cells 1:100 - 1:500.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00003Open
Product DatasheetPDF · IHC-00003 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00003 · Lot 1 (current lot)Open
Citations / Publications
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Resources
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