Bethyl Laboratories
Rabbit anti-AXL Recombinant Monoclonal Antibody [BLR222K]
Monoclonal Antibodies
Catalog #A700-222
TargetAXL
HostRabbit
CloneBLR222K
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IP, WB
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Product Description
AXL is a receptor tyrosine kinase (RTK) that binds the ligand Gas6 (growth-arrest specific gene). It is part of a sub-family of RTKs that includes AXL, SKY and MER. The AXL gene was first identified as a transforming cDNA isolated from patients with chronic myelogenous leukemia. AXL-Gas6 signaling is implicated in cellular responses such as cell survival, proliferation, migration, and adhesion, and it may be involved in cancer progression.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR222K
Host
Rabbit
Immunogen
Residues 33-440(ECD)
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetAXL
Antigen Species
Human
Gene ID558
Gene Symbol
AXL
Gene Name
AXL receptor tyrosine kinase
Uniprot IDP30530
Protein Name
Tyrosine-protein kinase receptor UFO
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human AXL (aka tyrosine-protein kinase receptor UFO; residues 33-440, NP_068713.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-222 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-222 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-222Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
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