Bethyl Laboratories
Rabbit anti-BAD IHC Antibody Affinity Purified
Primary
Catalog #IHC-00677
TargetBAD
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
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Product Description
BAD (Bcl2 agonist of cell death) is a member of the Bcl-2 family. Bcl-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein [taken from NCBI Entrez Gene (GeneID:572)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetBAD
Antigen Species
Human
Gene ID572
Gene Symbol
BAD
Gene Name
BCL2 associated agonist of cell death
Uniprot IDQ92934
Protein Name
Bcl2-associated agonist of cell death
Alternate Names
BAD • BBC2 • bcl2 antagonist of cell death • BCL2-antagonist of cell death protein • bcl2-associated agonist of cell death • bcl-2-binding component 6 • BCL2-binding component 6 • BCL2-binding protein • BCL2L8 • bcl2-L-8 • bcl-2-like protein 8 • BCL-X/BCL-2 binding protein • bcl-XL/Bcl-2-associated death promoter
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BAD immobilized on solid support.
The epitope recognized by IHC-00677 maps to a region between residue 1 and 50 of human BCL2-antagonist of cell death using the numbering given in entry NP_004313.1 (GeneID 572).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00677Open
Product DatasheetPDF · IHC-00677 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00677 · Lot 1 (current lot)Open
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