Bethyl Laboratories
Rabbit anti-BCAR1/p130CAS IHC Antibody Affinity Purified
Primary
Catalog #IHC-00598
TargetBCAR1/p130CAS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetBCAR1/p130CAS
Antigen Species
Human
Gene ID9564
Gene Symbol
BCAR1
Gene Name
BCAR1, Cas family scaffold protein
Uniprot IDP56945
Protein Name
Breast cancer anti-estrogen resistance protein 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BCAR1/p130CAS immobilized on solid support.
The epitope recognized by IHC-00598 maps to a region between residue 275 and 325 of human breast cancer anti-estrogen resistance 1 (Crk-associated substrate p130Cas) using the numbering given in entry NP_055382.2 (GeneID 9564).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00598Open
Product DatasheetPDF · IHC-00598 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00598 · Lot 1 (current lot)Open
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