Bethyl Laboratories
Rabbit anti-BCR IHC Antibody Affinity Purified
Primary
Catalog #IHC-00406
TargetBCR
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC, ICC-IF
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Product Description
BCR (breakpoint cluster region) was identified as part of a fusion protein that results from a reciprocal translocation with the ABL gene between chromosomes 22 and 9. The BCR-ABL fusion is the cause of chronic myeloid leukemia (CML). The BCR protein contains a C2 domain, a DBL-homology domain, a PH domain, and a Rho-GAP domain and functions as a GTPase-activating protein for RAC1 and CDC42.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetBCR
Antigen Species
Human
Gene ID613
Gene Symbol
BCR
Gene Name
BCR, RhoGEF and GTPase activating protein
Uniprot IDP11274
Protein Name
Breakpoint cluster region protein
Alternate Names
Product Information
Applications
ICC, ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BCR immobilized on solid support.
The epitope recognized by IHC-00406 maps to a region between residue 50 and 100 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended for formalin-fixed, paraffin-embedded cells.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunocytochemistry (ICC)1:100 - 1:500
Immunocytochemistry-Immunofluoresence (ICC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00406Open
Product DatasheetPDF · IHC-00406 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00406 · Lot 1 (current lot)Open
Services
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