Bethyl Laboratories
Rabbit anti-beta Catenin IHC Antibody Affinity Purified
Primary
Catalog #IHC-00584
Targetbeta Catenin
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
beta Catenin plays an integral role in the Wnt signaling pathway and is critical to morphogenic signaling and body axis specification. Beta Catenin exists in complexes with cadherins, transmembrane proteins that mediate cell adhesion. When Wnt-signaling proteins bind their receptors, beta Catenin is stabilized and accumulates in the cell. Translocation of stabilized beta Catenin allows its association with transcription factors and the activation of specific gene expression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 731 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
Targetbeta Catenin
Antigen Species
Human
Gene ID1499
Gene Symbol
CTNNB1
Gene Name
catenin beta 1
Uniprot IDP35222
Protein Name
Catenin beta-1
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to beta Catenin immobilized on solid support.
The epitope recognized by IHC-00584 maps to a region between residue 760 and 781 of human beta-catenin using the numbering given in entry NP_001895.1 (GeneID 1499).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00584Open
Product DatasheetPDF · IHC-00584 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00584 · Lot 1 (current lot)Open
Citations / Publications
Services
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