Bethyl Laboratories
Rabbit anti-Biotin Antibody Affinity Purified
Primary
Catalog #A150-109A
TargetBiotin
HostRabbit
ClonalityPolyclonal
ReactivitySpecies Independent
ApplicationELISA, ICC, IHC, WB
local_shipping
Order within 1 hours and 39 minutes for shipment by today
Product Description
Biotin, also known as Vitamin B7, is primarily known for its role in mammalian metabolism as a coenzyme. In molecular biology, biotin can be conjugated to molecules to serve as a binding partner for streptavidin.
Specifications
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Reactivity
Species Independent
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Target Identity
TargetBiotin
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with KLH-biotin and BSA-biotin. There is no reaction with free KLH or BSA.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A150-109AOpen
Product DatasheetPDF · A150-109A · Lot 260911 (current lot)Open
Safety Data SheetPDF · A150-109AOpen
Product DatasheetPDF · A150-109A · Lot 23Open
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1