Rabbit anti-Biotin Recombinant Monoclonal Antibody [BLR315M]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
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Immunogen was biotin conjugated to KLH.
Additional Product Information
Biotin, also known as Vitamin B7, is primarily known for its role in mammalian metabolism as a coenzyme. In molecular biology, biotin can be conjugated to molecules to serve as a binding partner for streptavidin.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.