/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Biotin Recombinant Monoclonal Antibody [BLR315M]
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Primary Antibodies  

Rabbit anti-Biotin Recombinant Monoclonal Antibody [BLR315M]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Biotin

Applications:

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR315M

Format:

Whole IgG

Immunogen:

Biotin

Isotype:

IgG

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Concentration
250 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


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Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free


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Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was biotin conjugated to KLH.

Additional Product Information

Biotin, also known as Vitamin B7, is primarily known for its role in mammalian metabolism as a coenzyme. In molecular biology, biotin can be conjugated to molecules to serve as a binding partner for streptavidin.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.