Bethyl Laboratories
Rabbit anti-BLM Recombinant Monoclonal Antibody [BLR312M]
Primary
Catalog #A700-312
TargetBLM
HostRabbit
CloneBLR312M
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Bloom syndrome protein (BLM) is a RecQ DNA-helicase that plays a role in DNA replication and repair. Mutations and inactivation of BLM result in human syndromes characterized by dwarfism, infertility, erythema, learning disabilities, immune deficiency and predisposition to cancer. BLM has been shown to associate with the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1, MSH2, MSH6, MLH1, ATM, PMS2 and the RAD50-MRE11-NBS1 protein complex.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR312M
Host
Rabbit
Immunogen
Between 1367 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetBLM
Antigen Species
Human
Gene ID641
Gene Symbol
BLM
Gene Name
Bloom syndrome RecQ like helicase
Uniprot IDP54132
Protein Name
Bloom syndrome protein
Alternate Names
Product Information
Applications
IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 1367 and the C-terminus (residue 1417) of human RecQ-like DNA helicase BLM using the numbering given in entry NP_000048.1 (Gene ID 641).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1,000
Documents
Product DatasheetPDF · A700-312 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-312 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-312Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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