Bethyl Laboratories
Rabbit anti-BORG2 Antibody Affinity Purified
Primary
Catalog #A301-723A
TargetBORG2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
BORG2 (binder of Rho GTPases 2) is a member of a family of proteins that bind the TC10 and Cdc42 GTPases in a GTP-dependent manner. BORG family members have been shown to play a role in the regulation of septin assembly and organization.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 204 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetBORG2
Antigen Species
Human
Gene ID10602
Gene Symbol
CDC42EP3
Gene Name
CDC42 effector protein 3
Uniprot IDQ9UKI2
Protein Name
Cdc42 effector protein 3
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BORG2 immobilized on solid support.
The epitope recognized by A301-723A maps to a region between residue 204 and 254 of human binder of Rho GTPases 2 using the numbering given in entry NP_006440.2 (GeneID 10602).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-723A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-723A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-723AOpen
Services
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