Bethyl Laboratories
Rabbit anti-BRAF IHC Antibody Affinity Purified
Primary
Catalog #IHC-00607
TargetBRAF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Image 1 of 1
local_shipping
Order within 20 hours and 14 minutes for shipment by Tuesday, November 3
Product Description
B-Raf proto-oncogene serine/threonine-protein kinase (BRAF) is a member of the raf/mil family of serine/threonine kinases. BRAF is involved in transducing mitogenic signals via the MAP kinase/ERKs signaling pathway. Mutations in BRAF are involved in a wide range of cancers such as lung, non-Hodgkin lymphoma, and colorectal cancer. Defects in BRAF are also the cause of cardiofaciocutaneous syndrome (CFC), an autosomal dominant syndrome characterized by facial anomalies, heart defects, and ectodermal abnormalities.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetBRAF
Antigen Species
Human
Gene ID673
Gene Symbol
BRAF
Gene Name
B-Raf proto-oncogene, serine/threonine kinase
Uniprot IDP15056
Protein Name
Serine/threonine-protein kinase B-raf
Alternate Names
94 kDa B-raf protein • B-raf • B-Raf proto-oncogene serine/threonine-protein kinase (p94) • B-Raf serine/threonine-protein • BRAF1 • B-RAF1 • murine sarcoma viral (v-raf) oncogene homolog B1 • NS7 • p94 • proto-oncogene B-Raf • RAFB1 • serine/threonine-protein kinase B-raf • v-raf murine sarcoma viral oncogene homolog B • v-raf murine sarcoma viral oncogene homolog B1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BRAF immobilized on solid support.
The epitope recognized by IHC-00607 maps to a region between residue 25 and 75 of human v-raf murine sarcoma viral oncogene homolog B1 using the numbering given in entry NP_004324.2 (GeneID 673).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00607Open
Product DatasheetPDF · IHC-00607 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00607 · Lot 1 (current lot)Open
Citations / Publications
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1