Rabbit anti-BRCA1 Recombinant Monoclonal Antibody [BLR319M]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 500 and 550 of mouse Breast cancer type 1 susceptibility protein (aka BRCA1) using the numbering given in entry NP_033894.3 (Gene ID 12189).
Additional Product Information
BRCA1 was identified as a gene with germline mutations that predisposes women to breast and ovarian cancer. The BRCA1 tumor suppressor is a large protein that has been demonstrated to interact with a multitude of proteins involved in the DNA damage response and cell cycle checkpoints to maintain genomic integrity. BRCA1 functions by regulating the activities of its interacting proteins which mediate processes such as gene transcription, gene repression, protein phosphorylation, and ubiquitination.
Alternate Names
breast cancer associated 1;breast cancer type 1 susceptibility protein homolog;RING-type E3 ubiquitin transferase BRCA1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.