Bethyl Laboratories
Rabbit anti-BRCA2 Antibody Affinity Purified
Primary
Catalog #A300-005A
TargetBRCA2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
BRCA2 was identified as a gene with germline mutations that predisposes women to breast cancer. An important function of BRCA2 involves its direct interaction with RAD51 and the process of homologous recombination in DNA double strand break repair. BRCA2 and BRCA1 primary sequences are not similar; however the proteins exhibit similar patterns of expression and sub-cellular localization. Although the phenotype of disruptions of BRCA2 is similar to BRCA1, BRCA2 appears to possess functions distinct from BRCA1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 3368 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetBRCA2
Antigen Species
Human
Gene ID675
Gene Symbol
BRCA2
Gene Name
BRCA2, DNA repair associated
Uniprot IDP51587
Protein Name
Breast cancer type 2 susceptibility protein
Alternate Names
BRCA1/BRCA2-containing complex, subunit 2 • BRCC2 • breast and ovarian cancer susceptibility gene, early onset • breast and ovarian cancer susceptibility protein 2 • breast cancer 2 tumor suppressor • breast cancer 2, early onset • breast cancer type 2 susceptibility protein • BROVCA2 • FACD • FAD • FAD1 • FANCD • FANCD1 • Fanconi anemia group D1 protein • GLM3 • mutant BRCA2 • PNCA2 • truncated breast cancer 2 • XRCC11
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BRCA2 immobilized on solid support.
The epitope recognized by A300-005A maps to a region between residue 3350 and the C-terminus (residue 3418) of human Breast Cancer Gene 2 using the numbering given in entry NP_000050.1 (GeneID 675).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A300-005A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-005A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-005AOpen
Citations / Publications
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