/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-BRD2 Antibody
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Primary Antibodies  

Rabbit anti-BRD2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (10)
Target:

BRD2

Reactivity:

Human

Applications:

ICC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to BRD2 immobilized on solid support.

The epitope recognized by A302-582A maps to a region between residue 1 and 50 of human bromodomain containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to BRD2 immobilized on solid support.

The epitope recognized by A302-582A-T maps to a region between residue 1 and 50 of human bromodomain containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).

Country of Origin

USA

Additional Product Information

BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle-regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements.

6046

BRD2

bromodomain containing 2

P25440

Bromodomain-containing protein 2

Alternate Names

BRD2 intronic transcript 1; BRD2-IT1; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSH; FSRG1; NAT; O27.1.1; really interesting new gene 3 protein; RING3; RNF3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.