Bethyl Laboratories
Rabbit anti-BRD2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00612
TargetBRD2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC
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Product Description
BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle-regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 700 and 750
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetBRD2
Antigen Species
Human
Gene ID6046
Gene Symbol
BRD2
Gene Name
bromodomain containing 2
Uniprot IDP25440
Protein Name
Bromodomain-containing protein 2
Alternate Names
Product Information
Applications
ICC-IF, IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BRD2 immobilized on solid support.
The epitope recognized by IHC-00612 maps to a region between residue 675 and 725 of human bromodomain containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Formaldehyde-Fixed Cells 1:100 - 1:500:
Permeabilization with Triton-X 100 is recommended.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00612Open
Product DatasheetPDF · IHC-00612 · Lot 3 (current lot)Open
Product DatasheetPDF · IHC-00612 · Lot 3 (current lot)Open
Citations / Publications
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Resources
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