/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-BRD9 Recombinant Monoclonal Antibody [BLR153J]
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Primary Antibodies  

Rabbit anti-BRD9 Recombinant Monoclonal Antibody [BLR153J]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

BRD9

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Platforms:

Simple Western

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR153J

Format:

Whole IgG

Immunogen:

Between 547 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

$127.00 $482.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 547 and the C-terminus (residue 597) of human Bromodomain-containing protein 9 using the numbering given in entry NP_076413.3 (Gene ID 65980).

Country of Origin

USA

Additional Product Information

BRD9 plays a role in chromatin remodeling and regulation of transcription. It acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. BRD9 is a component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. BRD9 also orchestrates RAD51-RAD54 complex formation and thereby plays a role in homologous recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H8M2].

65980

BRD9

bromodomain containing 9

Q9H8M2

Bromodomain-containing protein 9

Alternate Names

bromodomain-containing protein 9; LAVS3040; PRO9856; rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.