Bethyl Laboratories
Rabbit anti-BTF3 Antibody Affinity Purified
Primary
Catalog #A302-319A
TargetBTF3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Basic transcription factor 3 (BTF3) is a general transcription factor that forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20290].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetBTF3
Antigen Species
Human
Gene ID689
Gene Symbol
BTF3
Gene Name
basic transcription factor 3
Uniprot IDP20290
Protein Name
Transcription factor BTF3
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BTF3 immobilized on solid support.
The epitope recognized by A302-319A maps to a region between residue 35 and 70 of basic transcription factor 3 using the numbering given in entry NP_001032726.1 (GeneID 689).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-319A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-319A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-319AOpen
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