Bethyl Laboratories
Rabbit anti-BubR1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00498
TargetBubR1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
BubR1 is cell cycle checkpoint kinase and component of the spindle checkpoint machinery. BubR1 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. BubR1 is part of the mitotic checkpoint complex that also includes Bub3, Mad2, and Cdc20. In this trimer BubR1 functions to inhibit the anaphase promoting complex (APC) that promotes chromosome segregation. BubR1 is suggested to be a paralog of Bub1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetBubR1
Antigen Species
Human
Gene ID701
Gene Symbol
BUB1B
Gene Name
BUB1 mitotic checkpoint serine/threonine kinase B
Uniprot IDO60566
Protein Name
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Alternate Names
Bub1A • BUB1B, mitotic checkpoint serine/threonine kinase • BUB1beta • BUBR1 • budding uninhibited by benzimidazoles 1 homolog beta • hBUBR1 • MAD3/BUB1-related protein kinase • MAD3L • mitotic checkpoint kinase MAD3L • mitotic checkpoint serine/threonine-protein kinase BUB1 beta • MVA1 • Protein SSK1 • SSK1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BubR1 immobilized on solid support.
The epitope recognized by IHC-00498 maps to a region between residue 1 and 50 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in entry NP_001202.3 (GeneID 701).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00498Open
Product DatasheetPDF · IHC-00498 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00498 · Lot 1 (current lot)Open
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