Bethyl Laboratories

Rabbit anti-CA150 Antibody Affinity Purified

Primary
Catalog #A300-360A
TargetCA150
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
Detection of human CA150 by western blot.
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Product Description

CA150 has been identified as a transcriptional cofactor that functions as a corepressor of Tat-activated and C/EBP-activated gene transcription. CA150 also has been shown to interact with RNA polymerase II and the splicing factor SF1 to function in transcription elongation and pre-mRNA splicing.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCA150
Antigen Species
Human
Gene ID10915
Gene Symbol
TCERG1
Gene Name
transcription elongation regulator 1
Uniprot IDO14776
Protein Name
Transcription elongation regulator 1
Alternate Names
CA150  •  co-activator of 150 kDa  •  TAF2S  •  TATA box binding protein (TBP)-associated factor, RNA polymerase II, S, 150kD  •  TATA box-binding protein-associated factor 2S  •  transcription elongation regulator 1  •  transcription factor CA150  •  Urn1
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to CA150 immobilized on solid support.

The epitope recognized by A300-360A maps to a region between residues 1 and 50 of human Transcription Factor CA150 (Transcription Elongation Regulator 1) using the numbering given in entry NP_006697.2 (GeneID 10915).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:10,000

Documents

Product DatasheetPDF · A300-360A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-360A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-360AOpen

Citations / Publications

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