Bethyl Laboratories
Rabbit anti-cAbl Antibody Affinity Purified
Primary
Catalog #A301-660A
TargetcAbl
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC, IP, WB
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Product Description
c-Abl is the human cellular homolog of a the v-Abl viral oncogene. v-Abl was originally discovered as a mouse cell-derived sequence in the genome of the Abelson murine leukemia virus (A-MuLV), a transforming retrovirus isolated by the laboratory of Dr. H.T. Abelson. c-Abl is a tyrosine kinase from the Src-family of tyrosine kinases that localizes to both the cytoplasm and nucleus. It bears an SH2 (src-homology 2) and SH3 (src-homology 3) domain responsible for mediating c-Abl protein-protein interactions. c-Abl is implicated to play a role in multiple cellular processes such as cell cycle checkpoint signaling, apoptosis, cell differentiation, cell adhesion, and transcriptional regulation. Chromosomal translocations involving the c-Abl gene are associated with human leukemias.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetcAbl
Antigen Species
Human
Gene ID25
Gene Symbol
ABL1
Gene Name
ABL proto-oncogene 1, non-receptor tyrosine kinase
Uniprot IDP00519
Protein Name
Tyrosine-protein kinase ABL1
Alternate Names
Abelson murine leukemia viral oncogene homolog 1 • Abelson tyrosine-protein kinase 1 • ABL • bcr/abl • bcr/c-abl oncogene protein • c-ABL • c-abl oncogene 1, receptor tyrosine kinase • c-ABL1 • CHDSKM • JTK7 • p150 • proto-oncogene c-Abl • proto-oncogene tyrosine-protein kinase ABL1 • tyrosine-protein kinase ABL1 • v-abl • v-abl Abelson murine leukemia viral oncogene homolog 1
Product Information
Applications
ICC-IF, IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to cAbl immobilized on solid support.
The epitope recognized by A301-660A maps to a region between residue 550 and 600 of human v-abl Abelson murine leukemia viral oncogene homolog 1 using the numbering given in entry NP_005148.2 (GeneID 25).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For cytospin preparations of formaldehyde fixed cells permeabilization with Triton-X 100 is recommended.
Immunohistochemistry (IHC)1:100 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-660A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-660A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-660AOpen
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