Bethyl Laboratories
Rabbit anti-CaMKK alpha Antibody Affinity Purified
Primary
Catalog #A302-668A
TargetCaMKK alpha
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
CaMKK alpha is a calcium/calmodulin-dependent protein kinase that phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. CaMKK alpha is involved in regulating cell apoptosis. It promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N5S9].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCaMKK alpha
Antigen Species
Human
Gene ID84254
Gene Symbol
CAMKK1
Gene Name
calcium/calmodulin dependent protein kinase kinase 1
Uniprot IDQ8N5S9
Protein Name
Calcium/calmodulin-dependent protein kinase kinase 1
Alternate Names
calcium/calmodulin-dependent protein kinase kinase 1 • calcium/calmodulin-dependent protein kinase kinase 1, alpha • calcium/calmodulin-dependent protein kinase kinase alpha • caM-kinase IV kinase • caM-kinase kinase 1 • caM-kinase kinase alpha • caMKK 1 • caM-KK 1 • CaMKK alpha • caM-KK alpha • CAMKK alpha protein • CAMKKA
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CaMKK alpha immobilized on solid support.
The epitope recognized by A302-668A maps to a region between residue 150 and 200 of human calcium/calmodulin-dependent protein kinase kinase 1, alpha using the numbering given in entry NP_757343.1 (GeneID 84254).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-668A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-668A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-668AOpen
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