Bethyl Laboratories
Rabbit anti-CAND1 Antibody Affinity Purified
Primary
Catalog #A302-900A
TargetCAND1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Cullin-associated NEDD8-dissociated protein 1 (CAND1) is a regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. CAND1 prevents neddylation of CUL1 by physically blocking access to the neddylation site and disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins. CAND1 also binds TATA binding protein and enhances transcription from various promoters [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q86VP6].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 625 and 675
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCAND1
Antigen Species
Human
Gene ID55832
Gene Symbol
CAND1
Gene Name
cullin associated and neddylation dissociated 1
Uniprot IDQ86VP6
Protein Name
Cullin-associated NEDD8-dissociated protein 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CAND1 immobilized on solid support.
The epitope recognized by A302-900A maps to a region between residue 600 and 650 of human Cullin-Associated and Neddylation-Dissociated 1 using the numbering given in entry NP_060918.2 (GeneID 55832).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-900A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-900A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-900AOpen
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